Volume 6, Issue 2 (Fall And winter 2020)                   jpm 2020, 6(2): 42-34 | Back to browse issues page


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Turki H, Hoseini Z, Sarani M, Ghasemzadeh I, Ghanbarnejad A, Pournasrollah N et al . Efficacy of molecular method in detecting asymptomatic parasitic reservoirs of Malaria. jpm. 2020; 6 (2) :42-34
URL: http://jpm.hums.ac.ir/article-1-375-en.html
1- 1. Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2- Department of Medica Parasitology and Micology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
3- Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
4- HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
5- Department of Public Health, Social Determinants in Health Promotion Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
6- Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
7- MSC, Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Abstract:   (636 Views)
Introduction: Malaria Elimination Program Has Launched with the Technical Support from The World Health Organization Since 2007 in Iran. In Order to Achieve The Elimination of Malaria, All Positive Cases Should Be Diagnosed and Treated Promptly, Especially Asymptomatic and Low Parasitic cases. This Study Was Conducted to Determine The Effectiveness of The Molecular Method in The Detection of Asymptomatic Malaria Cases towards the successful Malaria Elimination Program in Iran.
Methods: In This Descriptive-analytical Study, 210 Samples Were Randomly Collected from Residents of High Risk malarious Areas of Hormozgan Province. The Rate of Asymptomatic Plasmodium Infection Was Evaluated Using Microscopic, RDT, and Nested-PCR Techniques (using18 ssrRNA).
Results: According to The Results, No Positive Asymptomatic Cases Were Observed with The Microscopic and RDT Methods, But by Using The Molecular Method, Three Positive Cases (1.4%) Were Detected.
Conclusion: The Results of This Study Showed That The sensitivity of molecular methods to detect asymptomatic parasitic reserviors is higher than other diagnostic methods and Nested-PCR is a good technique for detecting asymptomatic cases of malaria, therefore Use of a Sensitive Molecular Techniques With Wicroscopic and RDT Methods Is Necessary for The Detection of Asymptomatic Malaria Cases.
Key Words: Malaria, Detection, Parasitic Reservoir.
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.       Ashley EA, Pyae Phyo A, Woodrow CJ. Malaria. Lancet Infect Dis. 2018; 391(10130):1608-21. Doi: 10.1016/S0140-6736(18)30324-6
2.        Amirshekari MB, Nateghpour M, Raeisi A, Motevalli Haghi A, Farivar L, Edrissian G. Determination of asymptomatic Malaria among afghani and pakistani immigrants and native population in south of Kerman province, Iran J Parasitol. 2016; 11(2):247-52. PMID: 28096860
3.        Turki H, Raeisi A, Malekzadeh K, Ghanbarnejad A, Zoghi S, Yeryan M, et al. Efficiency of nested-PCR in detecting asymptomatic cases toward Malaria elimination program in an endemic area of Iran. Iran J Parasitol. 2015; 10(1):39-45. PMID: 25904944
4.        Tanner M, Greenwood B, Whitty CJ, Ansah EK, Price RN, Dondorp AM, et al. Malaria eradication and elimination: Views on how to translate a vision into reality. BMC Med. 2015;13:167. Doi: 10.1186/s12916-015-0384-6
5.        World Health Organization. World Malaria report 2018. Geneva: World Health Organization; 2018. Availabale at: https://www.who.int/Malaria/publications/world-Malaria-report-2018/en/
6.        Azizi MH, Bahadori M. Brief historical perspectives of Malaria in Iran. Arch Iran Med. 2013; 16(2):131. PMID: 23360639
7.        Edrissian G. Malaria in Iran: Past and present situation. IJP. 2006; 1(1):1-14.
8.        Norouzinejad F, Ghaffari F, Raeisi A. Epidemiological status of Malaria in Iran, 2011–2014. Asian Pac J Trop Med. 2016; 9(11):1055-61. Doi: 10.1016/j.apjtm.2016.09.007
9.        Turki H, Zoghi S, Mehrizi AA, Zakeri S, Raeisi A, Khazan H, et al. Absence of asymptomatic Malaria infection in endemic area of Bbashagard district, Hormozgan province, Iran. IJP. 2012; 7(1):36-44. PMID: 23133470
10.     Hassanpour G, Mohebali M, Zeraati H, Raeisi A, Keshavarz H. Asymptomatic Malaria and its challenges in the Malaria elimination program in Iran: A systematic review. J ArthropodBorne Dis. 2017 ;11(2):172-81. PMID: 29062842
11.     Lindblade KA, Steinhardt L, Samuels A, Kachur SP, Slutsker L. The silent threat: Asymptomatic parasitemia and Malaria transmission. Expert Rev Anti Infec. 2013; 11(6):623-39. Doi: 10.1586/eri.13.45
12.     Hemami MR, Sari AA, Raeisi A, Vatandoost H, Majdzadeh R. Malaria elimination in Iran, importance and challenges. IJPM. 2013; 4(1):88. PMID: 23413116
13.     Liu Y, Sturrock HJW, Yang H, Gosling RD, Cao J. The challenge of imported Malaria to eliminating countries. Lancet Infect Dis. 2017; 17(2):141. Doi: 10.1016/S1473-3099(17)30006-3
14.     Visser T, Daily J, Hotte N, Dolkart C, Cunningham J, Yadav P. Rapid diagnostic tests for Malaria. Bull World Health Organ. 2015; 93(12):862-6. Doi: 10.2471/BLT.14.151167
15.     Nateghpour M, Turki H, Keshavarz H, Edrissian GH, Mohebali M, Foroushani AR. A parasitological and serological study in Malaria suspected patients in Hormozgan province, southeastern Iran. IRCMJ. 2010; 12(3):242.
16.     Zimmerman PA, Howes RE. Malaria diagnosis for Malaria elimination. Curr Opin Infect Dis 2015;28(5):446-54. Doi: 10.1097/QCO.0000000000000191
17.     Ebrahimzadeh A, Dalir SN, Mirahmadi H, Mehravaran A, Khorashad AS, Turki H. The incidence of current infection with different human Malaria species by polymerase chain reaction for diagnosis of suspicious Malaria patients on elimination region Sistan and Baluchistan province, southeast of Iran. Jundishapur J Microbiol. 2017; 10(10): e58254. Doi: 10.5812/jjm.58254.
18.     World Health Organization. Basic Malaria microscopy. 2nd ed. Geneva: World Health Organization; 2010. Availabale at:  https://apps.who.int/iris/bitstream/handle/10665/44208/9789241547826_eng.pdf
19.     World Health Organization. Malaria rapid diagnostic tests Geneva: World Health Organization; 2017. Availabale at: https://apps.who.int/iris/bitstream/handle/10665/255038/9789241512275-eng.pdf
20.     Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN. Identification of the four human Malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasite. 1993; 58(2):283-92. Doi: 10.1016/0166-6851(93)90050-8
21.     Turki H, Rashid G, Shekari M, Raeisi A, Sharifi-Sarasiabi K. Malaria elimination program: Absence of asymptomatic Malaria and low parasitic in endemic area of Rudan district, Hormozgan Province, Iran. HMJ. 2017; 21(4):225-31.
22.     Roth JM, Korevaar DA, Leeflang MM, Mens PF. Molecular Malaria diagnostics: A systematic review and meta-analysis. Crit Rev Cl Lab Sci. 2016;53(2):87-105. Doi: 10.3109/10408363.2015.1084991
23.     Naeem MA, Ahmed S, Khan SA. Detection of asymptomatic carriers of Malaria in Kohat district of Pakistan. Malar J. 2018; 17(1):44. Doi: 10.1186/s12936-018-2191-y
24.     Baum E, Sattabongkot J, Sirichaisinthop J, Kiattibutr K, Jain A, Taghavian O, et al. Common asymptomatic and submicroscopic Malaria infections in Western Thailand revealed in longitudinal molecular and serological studies: A challenge to Malaria elimination. Malar J. 2016; 15(1):333. Doi: 10.1186/s12936-016-1393-4
25.     Maradiaga A, García JS, Mejía-Torres RE, Escober L, Matamoros J, Enríquez L, et al. Asymptomatic Malaria infections in an endemic city of Honduras. Hum Parasit Dis. 2016; 2016(8):37-41. Doi: 10.4137/HPD.s 40183
26.     Moreira CM, Abo-Shehada M, Price RN, Drakeley CJ. A systematic review of submicroscopic Plasmodium vivax infection. Malar J. 2015; 14(1):360. Doi: 10.1186/s12936-015-0884-z
27.     Recht J, Siqueira AM, Monteiro WM, Herrera SM, Herrera S, Lacerda MV. Malaria in Brazil, Colombia, Peru and Venezuela: Current challenges in Malaria control and elimination. Malar J. 2017; 16(1):273. Doi: 10.1186/s12936-017-1925-6
28.     De Andrade ALS, Martelli CM, Oliveira RM, Arias JR, Zicker F, Pang L. High prevalence of asymptomatic Malaria in gold mining areas in Brazil. Clin Infect Dis. 1995; 20(2):475. Doi: 10.1093/clinids/20.2.475
Type of Study: Research | Subject: Special
Received: 2020/03/15 | Accepted: 2020/03/15 | Published: 2020/03/15

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