Volume 12, Issue 3 (Autumn 2025)
J Prevent Med 2025, 12(3): 251-266 |
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Research code: 4010325
Ethics code: IR.HUMS.REC.1401.245
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Ahmadi K, Barazesh M, Mohammadi S, Kavousipour S. Comparative study of peptone production from fish waste in Bandar Abbas city using three methods of acid, alkaline and enzymatic hydrolysis. J Prevent Med 2025; 12 (3) :251-266
URL: http://jpm.hums.ac.ir/article-1-843-en.html
URL: http://jpm.hums.ac.ir/article-1-843-en.html
1- Department of Medical Biotechnology, Gerash University of Medical Sciences, Gerash, Iran
2- Department of Medical Biotechnology, Khorramabad University of Medical Sciences, Khorramabad, Iran
3- Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2- Department of Medical Biotechnology, Khorramabad University of Medical Sciences, Khorramabad, Iran
3- Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Abstract: (1071 Views)
Introduction: Optimizing the hygienic utilization of fish waste not only helps reduce environmental pollution but also plays a crucial role in maintaining public health and preventing diseases caused by the decomposition of such waste. This study aimed to produce peptone from native fish waste in Bandar Abbas and to compare different hydrolysis methods, including acidic, alkaline, and enzymatic approaches.
Methods: This study included three independent replicates for each treatment group (acidic, alkaline, and enzymatic hydrolysis). Fish waste samples consisting of viscera, skin, and muscle tissues were collected from native species in the Bandar Abbas region (Thunnus tonggol, Pampus argenteus, and various species of Lutjanus). For sample preparation, the materials were washed, defatted, homogenized using an industrial grinder, and subsequently subjected to hydrolysis. Acidic hydrolysis was performed using 1N HCl (pH ≈ 2, 100°C, 4 hours), while alkaline hydrolysis was carried out using 1N NaOH (pH = 11, 100°C, 4 hours). Enzymatic hydrolysis was conducted using Alcalase and trypsin under optimized temperature and pH conditions for 4 hours. Protein concentration was determined using the Biuret method, and the degree of hydrolysis was assessed by measuring trichloroacetic acid (TCA)-soluble peptides. Data were analyzed using one-way ANOVA followed by Duncan’s multiple range test for mean comparisons at a significance level of 0.05.
Results: Acidic hydrolysis resulted in the highest degree of hydrolysis (16.2 ± 1.2%) but the lowest protein concentration (30 ± 1.2 mg/mL). In contrast, alkaline hydrolysis showed a lower degree of hydrolysis (11.2 ± 2%) with a higher protein concentration (33 ± 1.5 mg/mL). Optimized enzymatic hydrolysis achieved the highest protein concentration (90 ± 2) after 24 hours. Functionally, peptones produced by alkaline and enzymatic hydrolysis demonstrated comparable performance to commercial peptones in supporting Escherichia coli growth, whereas peptones obtained through acidic hydrolysis supported reduced bacterial growth. These findings indicate that alkaline and enzymatic hydrolysis are effective methods for producing high-quality peptone from fish waste.
Conclusion: Alkaline and enzymatic hydrolysis are efficient methods for producing high-quality peptone from fish waste and may serve as cost-effective and environmentally friendly alternatives for industrial applications. This process not only reduces environmental pollution but also contributes to the sustainability of the fisheries industry and promotes the health of the local community.
Methods: This study included three independent replicates for each treatment group (acidic, alkaline, and enzymatic hydrolysis). Fish waste samples consisting of viscera, skin, and muscle tissues were collected from native species in the Bandar Abbas region (Thunnus tonggol, Pampus argenteus, and various species of Lutjanus). For sample preparation, the materials were washed, defatted, homogenized using an industrial grinder, and subsequently subjected to hydrolysis. Acidic hydrolysis was performed using 1N HCl (pH ≈ 2, 100°C, 4 hours), while alkaline hydrolysis was carried out using 1N NaOH (pH = 11, 100°C, 4 hours). Enzymatic hydrolysis was conducted using Alcalase and trypsin under optimized temperature and pH conditions for 4 hours. Protein concentration was determined using the Biuret method, and the degree of hydrolysis was assessed by measuring trichloroacetic acid (TCA)-soluble peptides. Data were analyzed using one-way ANOVA followed by Duncan’s multiple range test for mean comparisons at a significance level of 0.05.
Results: Acidic hydrolysis resulted in the highest degree of hydrolysis (16.2 ± 1.2%) but the lowest protein concentration (30 ± 1.2 mg/mL). In contrast, alkaline hydrolysis showed a lower degree of hydrolysis (11.2 ± 2%) with a higher protein concentration (33 ± 1.5 mg/mL). Optimized enzymatic hydrolysis achieved the highest protein concentration (90 ± 2) after 24 hours. Functionally, peptones produced by alkaline and enzymatic hydrolysis demonstrated comparable performance to commercial peptones in supporting Escherichia coli growth, whereas peptones obtained through acidic hydrolysis supported reduced bacterial growth. These findings indicate that alkaline and enzymatic hydrolysis are effective methods for producing high-quality peptone from fish waste.
Conclusion: Alkaline and enzymatic hydrolysis are efficient methods for producing high-quality peptone from fish waste and may serve as cost-effective and environmentally friendly alternatives for industrial applications. This process not only reduces environmental pollution but also contributes to the sustainability of the fisheries industry and promotes the health of the local community.
Type of Study: Orginal |
Subject:
Special
Received: 2025/07/31 | Accepted: 2025/11/2 | Published: 2026/08/11
Received: 2025/07/31 | Accepted: 2025/11/2 | Published: 2026/08/11
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